Journal: bioRxiv

Article Title: Early life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms

doi: 10.1101/369660

Figure Lengend Snippet: (A) The scheme illustrates the specificity of the oligodendrocyte lineage markers using triple immunostaining. Oligodendrocyte progenitor cells (OPCs) express Olig2 and high levels of PDGFRα while mature oligodendrocytes (OLs) express Olig2 and high levels of the CC1 antigen. Scale bar represents 20µm. (B, C, D, E) Graphs show the density of OPCs (B,D) and OLs (C,E) in the P15 (B,C n=5 animals per conditions) and adult (D,E n=5 animals per conditions) mPFCs of SFR and MS animals. (F) Scheme shows the protocol of sequential labelling of OPCs using IdU injections at P10 and BrdU injections at P15 to differentiate the fraction of P10 OPCs that are still cycling at P15 (blue, IdU + BrdU + ) from those which have started differentiation by then (purple, IdU + BrdU − ). (G) Graph shows an increased fraction of differentiated OPCs in P15 mPFC of MS animals as compared to SFR animals (n= 11 SFR animals and 10 MS animals). * p<0.05, ** p<0.01, *** p< 0.005.

Article Snippet: The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam, 205402, 1:1000), rat anti-Ctip2 monoclonal (Abcam, ab18465, 1:500), rabbit anti-cFos (Synaptic System 226003, 1:1000) for P9 MS/SFR pups, rabbit anti-cFos antiserum (Abcam, 190289, 1:1000) for DREADDs experiments, mouse anti-NeuN (US Biological, 1:600), rabbit anti-Olig2 (Millipore Ab9610, 1:1000), rat anti-PDGFRα (BD Bioscience, 558774, 1:600) and rabbit anti-Tbr1 (Abcam, 1:1000).

Techniques: Triple Immunostaining